Four natural epothilone derivatives isolated from Sorangium cellulosum strain So0157-2
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چکیده
منابع مشابه
A Novel Cold-Adapted Lipase from Sorangium cellulosum Strain So0157-2: Gene Cloning, Expression, and Enzymatic Characterization
Genome sequencing of cellulolytic myxobacterium Sorangium cellulosum reveals many open-reading frames (ORFs) encoding various degradation enzymes with low sequence similarity to those reported, but none of them has been characterized. In this paper, a predicted lipase gene (lipA) was cloned from S. cellulosum strain So0157-2 and characterized. lipA is 981-bp in size, encoding a polypeptide of 3...
متن کاملExtraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu
Complex environmental conditions can significantly affect bacterial genome size by unknown mechanisms. The So0157-2 strain of Sorangium cellulosum is an alkaline-adaptive epothilone producer that grows across a wide pH range. Here, we show that the genome of this strain is 14,782,125 base pairs, 1.75-megabases larger than the largest bacterial genome from S. cellulosum reported previously. The ...
متن کاملOrigins of Replication in Sorangium cellulosum and Microcystis aeruginosa
The genome of Sorangium cellulosum has recently been completely sequenced, and it is the largest bacterial genome sequenced so far. In their report, Schneiker et al. (in Complete genome sequence of the myxobacterium Sorangium cellulosum, Nat. Biotechnol., 2007, 25, 1281-1289) concluded that 'In the absence of the GC-skew inversion typically seen at the replication origin of bacterial chromosome...
متن کاملHeterologous expression of epothilone biosynthetic genes in Myxococcus xanthus.
Epothilones are potential anticancer drugs that stabilize microtubules in a manner similar to paclitaxel (Taxol). Epothilones are produced from the myxobacterium Sorangium cellulosum, which has a 16-h doubling time and produces only milligram-per-liter amounts of epothilone A and epothilone B. Furthermore, genetic manipulation of S. cellulosum is difficult. To produce epothilones in a more gene...
متن کاملNO formation by a catalytically self-sufficient bacterial nitric oxide synthase from Sorangium cellulosum.
The role of nitric oxide (NO) in the host response to infection and in cellular signaling is well established. Enzymatic synthesis of NO is catalyzed by the nitric oxide synthases (NOSs), which convert Arg into NO and citrulline using co-substrates O2 and NADPH. Mammalian NOS contains a flavin reductase domain (FAD and FMN) and a catalytic heme oxygenase domain (P450-type heme and tetrahydrobio...
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ژورنال
عنوان ژورنال: Journal of Chinese Pharmaceutical Sciences
سال: 2013
ISSN: 1003-1057
DOI: 10.5246/jcps.2013.01.002